Liquid Chromatography-Mass Spectrometry

Instrument: Thermo TSQ Endura MS with Dionex Ultimate 3000 RSLC; Thermo Exactive Plus Orbitrap MS with Dionex Ultimate 3000 RSLCnano

Analysis of pure solutions by mass spectrometry is typically done by direct infusion, however when the sample is composed of multiple components then an initial separation of those components is frequently useful and often essential. When some components of the sample interfere with MS analysis, such as inorganic salts and ionic buffers, these must be removed.

An extremely useful method of separating analytes, or of removing salts, is by chromatographic means. Typically HPLC or UPLC systems are used prior to mass spectrometric analysis and this is made easy by directly interfacing HPLC with MS, such that the output from the chromatographic column enters the MS directly.

LC-MS allows analytes from a mixture to be analysed by MS individually, thereby considerably simplifying data interpretation.
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