Recombinant Protein Production

Instrument: various pieces of equipment used

Life science research often requires proteins to be expressed in large quantities for functional and structural studies, for use in raising antibodies or for controls during immunoassays. We have access to incubators, equipment and extensive expertise to allow expression of proteins in either eukaryotic or prokaryotic cells. We can perform up-stream molecular biology and down-stream purification of the proteins, using our considerable infrastructure of chromatography systems. If refolds are required, we can investigate optimal conditions to yield well-folded proteins.

Once we have isolated your proteins, we can also perform initial or in depth characterisation, including functional assays and reports on secondary and tertiary structure. For small proteins, we have facilities to solve atomic-level structures by NMR methods. All protein reagents are characterised by polyacrylamide gel analysis and mass spectrometry, where appropriate.
Technical Detail
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