Proteomic and Metabolomic Workflows

Instrument: Dionex UltiMate 3000 Quaternary RSLC System with Dionex UltiMate 3000 Quaternary RSLCnano System

Overview
Proteomics involves predominately the high throughput identification, quantification and/or analysis of the primary structure of all proteins present in a given sample, although the term is also used to discribe extensive examination of the structure of indivudal proteins, such as the type, prevalence and structure of post translational modifications. In a similar vein, metabolomics is the study of the identity and concentrations of small molecule metabolites in a biological sample. 

Both proteomics and metabolomics typically involve mass spectrometric measurements, ideally using high resolution instruments, followed by multi-step data processing to identify molecular species, be they metabolites or proteins, and to extract quantitative information. Sophisticated network analysis tools can help inform the investigator which biochemical processes the identified proteins or metabolites are part of.

Proteomics and metabolomics pipelines provide a suite of tools that are important in the characterisation of biological processes, including analysis of drug responses, investigations of disease mechanisms or identification of gene function. Also useful in biomarker discovery, these pipelines can be used on samples as simple as purified proteins to those as complex as tissue lysates.
Technical Detail
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Sample requirements